Philippine Society of Biochemistry and Molecular Biology
  • Home
  • About
    • Constitution and By Laws
    • Board of Directors
    • Membership
    • Awards and Grants >
      • Awardees
      • Young Scientists' Forum
    • PSBMB Chapters >
      • North Luzon Chapter
      • Central Luzon Chapter
      • South Luzon Chapter
  • Conventions
    • 47th Annual Convention >
      • Abstract Submission
      • Registration
      • Scientific Program
      • Speakers
    • 46th Annual Convention
    • 45th Annual Convention
  • RevUp and REdUp
    • 1st REdUp
    • 18th Review and Updates
  • News
  • Newsletter
  • PJBMB
HOME
PROCESSING CHARGES
ARCHIVE
INDEXING
SPECIAL ISSUES
PUBLICATION ETHICS
NEWS
COPYRIGHT
EDITORIAL TEAM
CONTACT US
SUBMISSION & GUIDELINES
FAQs

Archive

1978, volume 1
  • June 1978, Vol. 1, Issue 1
  • ​December 1978, Vol. 1, Issue 2
1979, volume 2​
  • June 1979, Vol. 2, Issue 1
1980, volume 3
  • December 1980, Vol. 3, Issue 1 & 2
1981, volume 4
  • December 1981, Vol. 4, Issue 1 & 2
1982-1983, volume 5 & 6
  • 1982-1983, Vol. 5 & 6, Issue 1
1987, volume 7
  • June 1987, Vol. 7, Issue 1
  • December 1987, Vol. 7, Issue 2​
1988-1989, volume 8
  • Vol. 8, Issue 1
1990, volume 9
  • Vol. 9, Issue 1
1992-1993, VOLUME 11 & 12
  • Vol. 11 & 12, Issue 1​

​Cowpea lipoxygenase: Part 1 Physiochemical Characterization


The Bulletin of Philippine Biochemical Society
​Volume 2, Issue 1, 1979, Pages 1-12
​
Authors
Truong Van Den*, Leoncio C. Raymundo* and Evelyn Mae Tecson-Mendoza**
        
*Dept. of Food Science and Technology
**Institute of Plant Breeding, College of Agriculture, University of the Philippines - Los Baños, College, Laguna
 
Abstract
              Lipoxygenase (linoleate: oxygen oxidoreductase E.C.1.13.11.12) level was determined in ten varieties of cowpea, five varieties of soybean, and six varieties of winged bean. Cowpea lipoxygenase specific activity was highest (205 – 316 units/mg protein) as compared to that of winged bean (14 – 30 units/mg protein) and soybean (11- 48 units/mg protein).
          Crude cowpea lipoxygenase was observed to have optimum activity at about pH 6.2 and 30°C. The apparent Km and substrate concentration at Vmax of this enzyme which follows Michaelis-Menten kinetics were 0.195 x 10-3 and 1.6 x 10-3 M, respectively.
             Lipoxygenase activity was located in 40 – 50% and 50 – 60% ammonium sulfate precipitates with 3 and 5 fold enrichment in said fractions.
       Disc gel electrophoresis followed by specific enzyme staining revealed four isozymes of cowpea lipoxygenase with a major isozyme Rf = 0.21 – 0.27. The average molecular weight of this enzyme is 68,000 daltons as shown by Sephadex G – 50 gel chromatography.
         The possible role of lipoxygenase in the formation of the strong beany flavor of cowpea is discussed.
​ (1979)
 
To cite this article
V.A Truong, L.C. Raymundo and E.M. Tecson-Mendoza​, Cowpea lipoxygenase: Part 1 Physiochemical Characterization
, The Bulletin of Philippine Biochemical Society, Vol. 2, No. 1, 1979, pp. 1-12.      
Picture
Abstract

Picture
 ​ ​PDF


​SOCIETY PROJECTS
Journals
News
Conventions
RevUp
JOIN US
Apply as an Editor
Apply as an Editorial Board Member/Reviewer
Qualifications & Requirements
RESOURCES
Open access
For Authors
For Librarians
For Booksellers
Processing Charges
SPECIAL SERVICES
Download Certificates
Recommend to Library
Ordering from PJBMB
Subscribe
Unsubscribe
ADDRESS
Philippine Journal of Biochemistry and Molecular Biology
College of Medicine,
University of the Philippines
547 Pedro Gil St., Ermita
Metro Manila 1000
Tel: (02) 526 4197
Philippine Society of Biochemistry and Molecular Biology Copyright © 2017
  • Home
  • About
    • Constitution and By Laws
    • Board of Directors
    • Membership
    • Awards and Grants >
      • Awardees
      • Young Scientists' Forum
    • PSBMB Chapters >
      • North Luzon Chapter
      • Central Luzon Chapter
      • South Luzon Chapter
  • Conventions
    • 47th Annual Convention >
      • Abstract Submission
      • Registration
      • Scientific Program
      • Speakers
    • 46th Annual Convention
    • 45th Annual Convention
  • RevUp and REdUp
    • 1st REdUp
    • 18th Review and Updates
  • News
  • Newsletter
  • PJBMB