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Fractionation and Characterization of Ipil-Ipil (Leucaena leucocephala (Lam) de Wit.) Seed Proteins

The Bulletin of Philippine Biochemical Society
​Volume 4, Issue 1 & 2, 1981, Pages 15-32
                                      
Authors
R.L. Tantung and L.S.P. Madamba*

*Former graduate student, Dept. of Chemistry, UPLB, now Instructor, MSU-Sulu Development and Technical College, Jolo, Sulu 7601 and Assistant Professor, University of the Philippines - Los Baños, College, Laguna 3720, respectively.

Abstract
              Defatted meal from ipil-ipil seeds containing 34.40% crude protein was fractioned using the Osborne method. Total soluble proteins amounted to 79.8% which was broken down as follows: 51.7% albumins, 14.0% globulins, 3.9% prolamins and 10.0% glutelins. The residue contained 11.9% protein.
                All fractions except the prolamins were characterized for their physicochemical properties. Solubility tests showed that both albumins and globulins were least soluble at pH 7.0. When chromatographed through Sephadex G – 150, albumins exhibited two peaks (MW = 100,500). Electrophoretic properties revealed eleven bands for albumins, eight bands for globulins and two bands for glutelins. Polycarylamide gel electrophoresis in the presence of sodium dodecyl sulfate resolved eight bands for albumin (MW = 114,500).
               Amino acid profile indicated the presence of all essential amino acids. However, the amount of sulfur-containing amino acids and tryptophan were lower than those corresponding to the FAO protein pattern.

To cite this article
R.L. Tantung and L.S.P. Madamba, Fractionation and Characterization of Ipil-Ipil (Leucaena leucocephala (Lam) de Wit.) Seed Proteins, The Bulletin of Philippine Biochemical Society, Vol. 4, No. 1 & 2, 1981, pp. 15-32.     
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