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Preliminary Studies in Some Aspects of Amino Acid Biosynthesis in Juveniles of Penaeus monodon Fabricius: I - Incorporation of 14C from (U-14C) - Acetate into Amino Acids of Precipitable Proteins

The Bulletin of Philippine Biochemical Society
​Volume 3, Issue 1 & 2, 1980, Pages 12-22
                                      
Authors
Relicardo M. Coloso* and Lourdes J. Cruz**   

*Aquaculture Dept., Southeast Asian Fisheries Development Center, Tigbauan, Iloilo
**Dept. of Biochemistry, College of Medicine, University of the Philippines, Health Sciences Center, Metro Manila

Abstract
            Ion-exchange chromatography in conjunction with radioisotope labeling techniques were used to investigate the incorporation of 14C from (U-14C) – acetate into amino acids of precipitable proteins in P. monodon juveniles. Highest 14C radioactivity was found in the acidic amino acids, aspartic acid and glutamic acid. Little but significant radioactivity was observed in alanine, cysteine, glycine, proline and serine. Amino acids which incorporated little or no 14C were arginine, histidine, isoleucine lysine, methionine, phenylalanine, threonine, tyrosine and valine. It appears that P. monodon juveniles could not synthesize these amino acids from acetate. The essential nature of these amino acids in P. monodon is discussed.
​
To cite this article
R.M. Coloso and L.J. Cruz​, Preliminary Studies in Some Aspects of Amino Acid Biosynthesis in Juveniles of Penaeus monodon Fabricius: I - Incorporation of 14C from (U-14C) - Acetate into Amino Acids of Precipitable Proteins, The Bulletin of Philippine Biochemical Society, Vol. 3, No. 1 & 2, 1980, pp. 12-22.     
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