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Localization of Proteins and Enzymes in the Granules of Conus Venom

The Bulletin of Philippine Biochemical Society
​Volume 4, Issue 1 & 2, 1981, Pages 33-44
                                      
Authors
Elsie C. Jimenez* and Lourdes J. Cruz**

*UP College Baguio, Baguio City
**Dept. of Biochemistry and Molecular Biology, College of Medicine, Health Sciences Center, University of the Philippines, Manila and Marine Sciences Center, UP Quezon City.

Abstract           
                The toxicity and enzyme activities of granules and soluble fraction extracted from various segments of the venom duct of Conus striatus were studied. Examinations reveal the granules to contain 60 to 67% of the total proteins. Using fish as assay animal, the soluble fraction was found to possess greater toxicity accounting for 63 to 73% of the total paralytic and lethal activities. Higher enzyme activities are associated with the granules which contain 74 to 85% of the total proteolytic activity, 80 to 84% of the total acetylcholinesterase activity and 90 to 94% of the total phosphodiesterase activity. Total proteins in the granules were noted to increase towards the posterior duct region except in the segment closest to the venom bulb while those in the soluble fraction increase from the anterior to the posterior region. Total paralytic and lethal activities were observed to increase towards the posterior duct region. Total enzyme activities increase from the anterior to the posterior region except in the segment adjacent to the venom bulb.
                Treatment of the granules extracted from the entire venom duct with carboxypeptidase A led to the enhancement of paralytic activity. Electrophoretic patterns in polyacrylamide gels of aliquots withdrawn before and after incubation of the granules with carboxypeptidase A have shown difference in mobility of a major protein.
                The results support the suggested role of granules as storage packets for enzymes and for inactive precursors of the toxic components of Conus venoms.

To cite this article
E.C. Jimenez and L.J. Cruz, Localization of Proteins and Enzymes in The Granules of Conus Venom, The Bulletin of Philippine Biochemical Society, Vol. 4, No. 1 & 2, 1981, pp. 33-44.     
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