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Radioimmunoassay of LH in the Carabao

The Bulletin of Philippine Biochemical Society
​Volume 7, Issue 2, 1987, Pages 51-56
                                                          
Authors
A. Alejandrino*, J. Alcantara, E. Wagelie, C. Goze, J. Sevilla, A. Caburian and C. Oprenario

A Alejandrino, Senior Researcher and 1985 PAEC Researcher of the Year Awardee for this work; E Wagelie, former head of the National Animal Breeding Center and Professor of Animal Science at UPLB; J Alcantara, research veterinarian at the Bureau of Animal Industry; C. Goze, J. Sevilla, A. Caburian and C. Oprenario are biomedical researchers at PAEC
*To whom correspondence should be addressed

Abstract                                              
         A sensitive radioimmunoassay method for the measurement of luteinizing hormone (LH) in carabao serum was validated. LH surge concentrations in 21 confined female carabaos were determined.
           Majority of the animals showed the preovulatory LH peak. Twelve of the 21 carabaos (57.1%) had high values (5 ng/mL or greater); others gave medium or low values. Basal, tonic values (0.4 ± 0.2 ng/mL) were observed during the estrous cycle except for the brief LH surge which appeared at either early or late estrus stage. During pregnancy, LH concentrations also remained basal (0.5 ± 0.3 ng/mL), apparently suppressed by the sustained higher levels of progesterone.
        The time of LH peak appearance and intensity relative to onset of estrus are useful in selecting carabaos which ovulate normally. Optimum time of insemination can also be determined for a successful breeding program. Carabaos with a late LH surge during estrus require another insemination 96 h after prostaglandin treatment to improve the conception rate. LH profiles can also be used to evaluate reproductive problems related to ovulation.
​
To cite this article
A. Alejandrino, J. Alcantara, E. Wagelie, C. Goze, J. Sevilla, A. Caburian and C. Oprenario​, Partial Radioimmunoassay of LH in the Carabao, The Bulletin of Philippine Biochemical Society, Vol. 7, No. 2, 1987, pp. 51-56. 
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