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Alpha-Amylase Production in Shake Flask Culture of Bacillus subtilis NRRL 3411

The Bulletin of Philippine Biochemical Society
​Volume 4, Issue 1 & 2, 1981, Pages 45-55
                                      
Authors
Claudia V. Valdeavella and Ernesto J. del Rosario*

*Dept. of Chemistry, College of Arts and Sciences and National Institute of Biotechnology and Applied Microbiology (BIOTECH), University of the Philippines - Los Baños, College, Laguna.
             
Abstract           
                Bacillus subtilis NRRL B3411 was found to give the highest yield of alpha-amylase in shake-flask culture in comparison with Bacillus licheniformis NRRL 1001 and a local isolate of Bacillus amyloliquefaciens. The cultural and nutritional requirements for alpha-amylase production by this organism were determined. Peptone was the ideal nitrogen source. Optimal values of the pH, temperature, and period of incubation for alpha-amylase formation were 6.5-7.5, 40°C, and five days, respectively.

To cite this article
C.V. Valdeavella and E.J. del Rosario, Alpha-Amylase Production in Shake Flask Culture of Bacillus subtilis NRRL 3411, The Bulletin of Philippine Biochemical Society, Vol. 4, No. 1 & 2, 1981, pp. 45-55.  
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