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Biochemical Characterization of Some Toxins from Conus striatus Venom

The Bulletin of Philippine Biochemical Society
​Volume 5 & 6, Issue 1, 1982 - 1983, Pages 15-24
                                      
Authors
Marcelina B. Lirazan* and Lourdes J. Cruz**

*Dept. of Chemistry, University of the East, Claro M. Recto, Manila
**Dept. of Biochemistry and Molecular Biology, College of Medicine, UP Manila and Marine Sciences Center, UP Diliman, Quezon City.
             
Abstract                
                Crude venom fom C. striatus was found to have an LD50 of 8.5 µg protein per gram mouse injected intraperitoneally and an LD50 of 0.8 µg protein per gram carp injected intramuscularly. The minimum paralysis and death times for mice were estimated to be 1.9 and 4.8 min and for carps 0.5 and 3.5 min, respectively.
        Using fish for bioassay, three toxins were resolved from the venom extract by phosphocellulose chromatography. Lethal peaks eluting at 0.01M, 0.2M and 0.27M of N-ethylmorpholine acetate buffer which accounted for 7%, 41% and 52% of total activity recovered from the column were designated as Toxins I, II and III respectively. Toxin I appears to be a small acidic peptide (Mr < 1500). On the other hand, Toxins II and III behaved as basic polypeptides with molecular weights of around 10,000 to 14,000 daltons. All toxins were found to be sensitive to heat and ß-mercaptoethanol.

To cite this article
M.B. Lirazan and L.J. Cruz, Biochemical Characterization of Some Toxins from Conus striatus Venom, The Bulletin of Philippine Biochemical Society, Vol. 5 & 6, No. 1, 1982- 1983, pp. 15-24.  
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