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Effect of Ascorbic Acid on Saccharin-Enhanced Mutagenicity of Mitomycin C

The Bulletin of Philippine Biochemical Society
​Volume 11 & 12, Issue 1, 1992-1993, Pages 26-29
                                                
Authors
Leandro C. Dimayuga, Julius C. Dalupang, Chona Diokno, Raymundo Dimaculangan, H.U. Barcelon, A.O Cantilep, M.T. Cortes, J.F. David, G.A. del Castillo, M.V. Demetria and Dalisay C. Sy

Dept. of Biochemistry, Faculty of Medicine and Surgery, University of Santo Tomas, Manila
​
Abstract                                                                                       
      Effectiveness of ascorbic acid in inhibiting the enhancing effect of saccharin on the mutagenicity of mitomycin C was demonstrated. Eight to nine weeks old Strong A male mice were injected i.p. with mitomycin C at a concentration of 3 mg/Kg BW. This was followed 3h after by i.p. injection of saccharin, 2.5 g/Kg BW. Two groups of experimental animals were utilized: one, receiving ascorbic acid 1h after saccharin injection and the second group, 1h before administration of saccharin. Both groups were sacrificed 30h after mitomycin C treatment and bone marrow cells isolated following Schmid’s procedure. Cells were smeared, stained with May Grunwald Giemsa and scored for micronuclei. Animals that received ascorbic acid prior to saccharin treatment showed 48% reduction in the number of micronuclei. In contrast, animals that received the vitamin after saccharin injection showed 3.87% reduction. Results suggest the effectivity of ascorbic acid in diminishing the effect of mitomycin C when the vitamin is given before but not after saccharin administration.

Keywords: Mitomycin C, mutagenicity, ascorbic acid, saccharin.

To cite this article
L.C. Dimayuga, J.C. Dalupang, C. Diokno, R. Dimaculangan, H.U. Barcelon, A.O Cantilep, M.T. Cortes, J.F. David, G.A. del Castillo, M.V. Demetria and D.C. Sy, Effect of Ascorbic Acid on Saccharin-Enhanced Mutagenicity of Mitomycin C, The Bulletin of Philippine Biochemical Society, Vol. 11 & 12, No. 1, 1992-1993, pp.  26-29. 
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