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Fed-Batch Cellulase Production by Trichoderma and Penicillium Strains

The Bulletin of Philippine Biochemical Society
​Volume 7, Issue 1, 1987, Pages 20-26
                                                          
Authors
Estrella P. Ocreto** and Ernesto J. del Rosario*

*Institute of Chemistry, University of the Philippines - Los Baños, College, Laguna
**Dept. of Chemistry, University of Southern Mindanao, Cotabato
​
Abstract                                 
                Fed-batch cellulase production by Trichoderma reesei NRRL 11460, Trichoderma viride PxF, and Penicillium sp. 86 (a local isolate) was studied using corn cobs as carbon source with pH controlled at 5.0 in a 3.5 liter airlift fermenter. Of the three fungal strains tested, T. reesei 11460 showed the highest volumetric activities, i.e., 1.34 I.U./mL on filter paper and 13.38 I.U./mL on CM-cellulose at a total substrate level of 80 g/L after 9.5 days of fermentation.
          Fed-batch cellullase production by T. reesei 11460 was further studied using “Bagras” (Eucalyptus deglupta Blume) and “Putian” (Alangium meyeri Merr) wood pulp as substrate. When the organism was cultured on “Putian” wood pulp, maximum volumetric activities were obtained after 10 days of fermentation, namely 3.53 I.U./mL on filter paper and 63.36 I.U./mL on CM-cellulose. Maximal specific activities of 0.84 I.U./mg and  12.10 I.U./mg were observed using filter paper and CM-cellulose as substrate, respectively, after 1-2 days fermentation.

To cite this article
E.P. Ocreto and E.J. del Rosario​, Fed-Batch Cellulase Production by Trichoderma and Penicillium Strains, The Bulletin of Philippine Biochemical Society, Vol. 7, No. 1, 1987, pp. 20-26.  
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Abstract
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