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Hydrolysis of Coconut Endosperm Carbohydrate by a Microbial Mannanase

The Bulletin of Philippine Biochemical Society
​Volume 2, Issue 1, 1979, Pages 22-29
​
Authors
Veronica J. Papa and Ernesto J. del Rosario*
       
*Dept. of Chemistry, College of Arts & Sciences, University of the Philippines - Los Baños, College, Laguna 3720
 
Abstract
           A crude mannanase was produced by a microbial isolate, which probably belongs to the genus Micrococcus, using coconut endosperm fibrous residue as substrate. Enzyme production was maximum at pH 7.5 and after four days of incubation at room temperature (~29°C). The optimal pH and temperature for enzyme assay were 6.4 and 50°C, respectively. The enzyme was stable in the pH range of 6.0 – 6.8 and its activity rapidly decreased at a temperature greater than 50°C. it converted 40% of the dry weight of the coconut residue into reducing sugars at pH 6.4 and 50°C after one hour of incubation. Precipitation with ammonium sulfate at 60 – 70% saturation resulted in a 2.4 – fold purification of the enzyme.
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To cite this article
V.J. Papa and E.J. del Rosario, Hydrolysis of Coconut Endosperm Carbohydrate by a Microbial Mannanase, The Bulletin of Philippine Biochemical Society, Vol. 2, No. 1, 1979, pp. 22-29.     
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