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Preliminary Evaluation of rbcL, matK, and SRAP Markers for the Molecular Characterization of Five Philippine Allium sativum varieties

Philippine Journal of Biochemistry and Molecular Biology
2020; 1(1&2): 54-60
Authors
Bigtas, Allisandra Isabel B., Moreno, Patrick Gabriel G., and Heralde, Francisco III M.
Abstract
Allium sativum, commonly known as “bawang”, is one of the economically important crops in the Philippines mainly for spice and other uses including herbal medicine for hypertension and cancer. Several studies showing therapeutic properties of garlic often do not disclose the variety used while typical varietal identification rely on phenotypic or agronomic traits which may be inadequate to define distinct variants. This study aimed to evaluate the utility of chloroplast genes, rbcL and matK, as well as Sequence-Related Amplified Polymorphism (SRAP) to define and characterize five A. sativum varieties grown in the Philippines. Five garlic varieties (i.e., GNT4, GMX6, IP3A, GMR10 and GTB17) were collected from a demo farm in Batac, Ilocos Norte, Philippines. The bulbs were made to grow roots which were subsequently used for DNA extraction and PCR analysis. Results showed that PCR amplified sequences of rbcL and matK genes were able to identify A. sativum varieties with ample discriminatory power to differentiate closely related species. The identified SRAP markers were also able to show a degree of polymorphism among the five local varieties. The rbcL sequences were highly conserved, the matK-sequences were able to discriminate GTB17 while the SRAP using the primer combinations ME1-EM3 and ME3- EM3 discriminated all the five varieties. A summary of the concatenated molecular features of the five varieties was provided. Albeit preliminary, the study was able to show the potential of matK and SRAP in discriminating the local A. sativum varieties from each other and from other varieties of different origin.
KEYWORDS: Allium sativum, molecular characterization, rbcL, matK, SRAP
To cite: Bigtas AIB, Moreno Patrick GG., and Heralde FIIIM.2020. Preliminary Evaluation of rbcL, matK, and SRAP Markers for the Molecular Characterization of Five Philippine Allium sativum varieties. PJBMB. Vol. I, No. 1, 2020, pp. 54-60. doi: 10.5555/pjbmb.ph.2020.01.01.54
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